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KMID : 0364219900330030266
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Korean Journal of Zoology
1990 Volume.33 No. 3 p.266 ~ p.275
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Immunocytochemical Localization of c-raf Protein Kinase in EC-4 Cell
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Choi Won-Chul
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Abstract
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c-raf protein kinase, a kind of oncogene, is a cytopiasmic serine / threonine-specific protein and is activated by mitogenic or oncogenic signals. The strncture and functions of c-raf protein kinase are considered very similar to those of protein kinase C. Using immunocytochemical approach, the time course of singal transduction of c-raf protein kinase in EC-4 cell was examined with 12-0-tetradecanoylphorbol-13-acetate (TPA) as tumor promotor and plateletderived growth factor (PDGF) as mitogenic factor. Immunoreactive c-raf was initially bound to the perinuclear membrane and then moved into the nucleus. The effect of the long-term treatment with TPA or PDGF was taken place down regulation at different time point. These results indicate that TPA and PDGF give rise to the translocation of c-raf protein kinase through the two different pathways.
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KEYWORD
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c-raf protein kinase , TPA , PDGF , Signal transduction , Down regulation
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